LC3A/B Antibody 14 Publications

Mouse Monoclonal| Catalog number: 66139-1-Ig |CloneNo.: 2B2B5

  • Print page
  • Download PDF

Species specificity:
human, pig, mouse, hamster, rat

Positive WB detected in:
human brain tissue, HeLa/HepG2 cells

Positive IHC detected in:
human skeletal muscle tissue, human liver tissue, human skeletal muscle tissue

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:200

Product Information


Source:
Mouse

Purification method:
Protein G purification

Isotype:
IgG1

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Fusion Protein

Full name:
microtubule-associated protein 1 light chain 3 alpha

Calculated molecular weight:
14 kDa

Observed molecular weight:
14 kDa, 16 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
MAP1LC3A

Synonyms
LC3, LC3A, LC3A/B, LC3B, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, MAP1ALC3, MAP1BLC3, MAP1LC3A, MAP1LC3A/B, MAP1LC3B
Background

LC3, also known as microtubule-associated protein 1 light chain 3 (MAP1LC3), is a mammalian homolog of the yeast Atg8p (autophagy-related 8 protein) that is involved in autophagy process. Three isoforms of LC3 exist as LC3A,LC3B, and LC3C. LC3 is first synthesized as pro-LC3, and undergoes C-terminal cleavage to yield a cytosolic precursor known as LC3-I. During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I and LC3-II.


Back
to top