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- KD/KO Validated
LDHA-Specific Polyclonal antibody
LDHA-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
FC, IF, IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
182
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, Jurkat cells, mouse testis tissue, rat kidney tissue, A431 cells, HeLa cells, NIH/3T3 cells, MCF-7 cells |
Positive IP detected in | mouse kidney tissue |
Positive IHC detected in | human heart tissue, human skeletal muscle tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
19987-1-AP targets LDHA-Specific in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF, IHC, IP, WB, ELISA |
Cited Applications | CoIP, FC, IF, IHC, IP, RIP, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, goat, chicken |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | lactate dehydrogenase A |
Synonyms | lactate dehydrogenase A, LDH A, LDH M, LDH muscle subunit, LDH1, LDHA, LDHA-Specific, PIG19 |
Calculated Molecular Weight | 37 kDa |
Observed Molecular Weight | 32-37 kDa |
GenBank Accession Number | NM_005566 |
Gene Symbol | LDHA |
Gene ID (NCBI) | 3939 |
RRID | AB_10646429 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P00338 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for LDHA-Specific antibody 19987-1-AP | Download protocol |
IHC protocol for LDHA-Specific antibody 19987-1-AP | Download protocol |
IF protocol for LDHA-Specific antibody 19987-1-AP | Download protocol |
IP protocol for LDHA-Specific antibody 19987-1-AP | Download protocol |
FC protocol for LDHA-Specific antibody 19987-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis | ||
Drug Resist Updat Targeting ACYP1-mediated glycolysis reverses lenvatinib resistance and restricts hepatocellular carcinoma progression
| ||
Nat Immunol NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death. | ||
Cell Metab Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export. | ||
ACS Nano Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy. |