MRPL44 Polyclonal antibody

MRPL44 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 16394-1-AP

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Synonyms

L44MT, MRP L44, MRPL44



经过测试的应用

Positive WB detected inA549 cells, rat brain tissue, HeLa cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16394-1-AP targets MRPL44 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited Applications IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Immunogen MRPL44 fusion protein Ag9485 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein L44
Synonyms L44MT, MRP L44, MRPL44
Calculated Molecular Weight 332 aa, 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC012058
Gene Symbol MRPL44
Gene ID (NCBI) 65080
RRIDAB_2146062
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H9J2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRP-L44 (mitochondrial ribosomal protein L44) is a 332 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 39S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis.

实验方案

Product Specific Protocols
WB protocol for MRPL44 antibody 16394-1-APDownload protocol
IHC protocol for MRPL44 antibody 16394-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

An in vitro system to silence mitochondrial gene expression.

Authors - Luis Daniel Cruz-Zaragoza
monkeyWB

Nat Cell Biol

MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.

Authors - Tim König
mouseWB

Sci Adv

Fidelity of translation initiation is required for coordinated respiratory complex assembly.

Authors - Danielle L Rudler
mouseWB

Sci Adv

Adult-onset obesity is triggered by impaired mitochondrial gene expression.

Authors - Kara L Perks
humanWB

Cell Metab

The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression.

Authors - Antonicka Hana H
humanWB

Nucleic Acids Res

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Authors - Elena Lavdovskaia