Mouse IL1-beta ELISA Kit4 Publications

Catalog number: KE10003



96 T


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Product name:
Mouse IL1-beta ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant, Cell lysate

Assay type:

1.0 pg/mL

7.8-500 pg/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Cell culture supernatant 113% 89%-126%
Cell lysate 104% 82%-128%
Mouse serum 102% 91%-123%

Samplenmean (pg/mL)SDCV%
1 20 520.0 17.0 3.3
2 20 159.5 5.6 3.5
3 20 29.1 0.5 1.8

Samplenmean (pg/mL)SDCV%
1 24 485.5 20.1 4.1
2 24 156.6 4.8 3.1
3 24 28.5 1.3 4.6

Product overview:
KE10003 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-1β ELISA kit is to be used to detect and quantify protein levels of endogenous IL-1β. The assay recognizes mouse IL-1β. A monoclonal antibody specific for IL-1β has been pre-coated onto the microwells. The IL-1β protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for IL-1β is added to detect the captured IL-1β protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.
NB: presursor and active form can be detected by this kit.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Il 1b, IL 1beta, IL-1, Il1b, IL-1b, interleukin 1 beta

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor IL-1RN. Interleukin 1β(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.

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