• Featured Product
  • KD/KO Validated

NUDT21 Polyclonal antibody

NUDT21 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 10322-1-AP

Print datasheet

Synonyms

CFIM25, CPSF 25 kDa subunit, CPSF25, CPSF5, Nudix motif 21, NUDT21



经过测试的应用

Positive WB detected inHL-60 cells, NIH/3T3 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NUDT21 Ready-To-Use IHC Kit. NUDT21 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10322-1-AP targets NUDT21 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited ApplicationsChIP, CoIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen NUDT21 fusion protein Ag0393 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 21
Synonyms CFIM25, CPSF 25 kDa subunit, CPSF25, CPSF5, Nudix motif 21, NUDT21
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC001403
Gene Symbol NUDT21
Gene ID (NCBI) 11051
RRIDAB_2251496
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43809
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.

实验方案

Product Specific Protocols
WB protocol for NUDT21 antibody 10322-1-APDownload protocol
IHC protocol for NUDT21 antibody 10322-1-APDownload protocol
IF protocol for NUDT21 antibody 10322-1-APDownload protocol
IP protocol for NUDT21 antibody 10322-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.

Authors - Chioniso P Masamha
  • KD Validated
humanWB

Mol Cell

A Complex of U1 snRNP with Cleavage and Polyadenylation Factors Controls Telescripting, Regulating mRNA Transcription in Human Cells.

Authors - Byung Ran So
  • KD Validated
WB

Mol Cell

Global Promotion of Alternative Internal Exon Usage by mRNA 3' End Formation Factors.

Authors - Ashish Misra
humanWB

Sci Adv

Alternative polyadenylation is a determinant of oncogenic Ras function.

Authors - Aishwarya Subramanian
humanIHC

J Exp Med

Downregulation of CFIm25 amplifies dermal fibrosis through alternative polyadenylation.

Authors - Tingting Weng
mouseWB

EMBO J

The Mediator subunit MED23 couples H2B mono-ubiquitination to transcriptional control and cell fate determination.

Authors - Xiao Yao