• Featured Product
  • KD/KO Validated

OS9 Polyclonal antibody

OS9 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 10061-1-AP

Print datasheet

Synonyms

Amplified in osteosarcoma 9, OS 9, OS9, Protein OS 9



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10061-1-AP targets OS9 in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, WB,ELISA
Cited Applications IF, WB
Tested Reactivity human
Cited Reactivityhuman
Immunogen OS9 fusion protein Ag0106 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name osteosarcoma amplified 9, endoplasmic reticulum associated protein
Synonyms Amplified in osteosarcoma 9, OS 9, OS9, Protein OS 9
Calculated Molecular Weight 76 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC000532
Gene Symbol OS9
Gene ID (NCBI) 10956
RRIDAB_2236537
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13438
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OS-9 is a ubiquitously expressed ensoplasmic reticulum (ER)-associated protein originally identified as being amplified in certain osteosarcomas. It functions in ER quality control and ER-associated degradation (ERAD). There are three isoforms of this protein: OS-9.1, OS-9.2 and OS-9.3. The longest isoform, OS-9.1, contains 667 aa, OS-9.2 lacks aa 535-589, whereas OS-9.3 lacks aa 456-470 and 535-589. OS-9.1 and OS-9.2 are N-glycosylated, ubiquitously expressed in human tissues, and amplified in tumors. Isoform 2 is the major isoform detected in all cell types examined. This antibody can recognize all three isoforms of OS-9.

实验方案

Product Specific Protocols
WB protocol for OS9 antibody 10061-1-APDownload protocol
IHC protocol for OS9 antibody 10061-1-APDownload protocol
IF protocol for OS9 antibody 10061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

OS-9 and GRP94 deliver mutant alpha1-antitrypsin to the Hrd1-SEL1L ubiquitin ligase complex for ERAD.

Authors - Christianson John C JC
  • KD Validated
humanWB,IF

J Biol Chem

OS-9 regulates the transit and polyubiquitination of TRPV4 in the endoplasmic reticulum.

Authors - Wang Yan Y
  • KD Validated
humanWB

J Biol Chem

Human OS-9, a lectin required for glycoprotein endoplasmic reticulum-associated degradation, recognizes mannose-trimmed N-glycans.

Authors - Hosokawa Nobuko N
humanWB

J Biol Chem

Human XTP3-B forms an endoplasmic reticulum quality control scaffold with the HRD1-SEL1L ubiquitin ligase complex and BiP.

Authors - Hosokawa Nobuko N
humanWB

J Biol Chem

SEL1L protein critically determines the stability of the HRD1-SEL1L endoplasmic reticulum-associated degradation (ERAD) complex to optimize the degradation kinetics of ERAD substrates.

Authors - Iida Yasutaka Y
  • KD Validated
humanWB

FEBS J

Endoplasmic reticulum lectin XTP3-B inhibits endoplasmic reticulum-associated degradation of a misfolded α1-antitrypsin variant.

Authors - Fujimori Tsutomu T
  • KD Validated