- Featured Product
- KD/KO Validated
PAN2 Polyclonal antibody
PAN2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, HepG2 cells |
| Positive IHC detected in | mouse heart tissue, human heart tissue, human kidney tissue, human placenta tissue, human testis tissue, human brain tissue, human liver tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | NIH/3T3 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16427-1-AP targets PAN2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | IF, IHC, IP, WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | PAN2 fusion protein Ag9670 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) |
| Synonyms | hPAN2, KIAA0710, PAN2, USP52 |
| Calculated Molecular Weight | 1198 aa, 135 kDa |
| Observed Molecular Weight | 135 kDa |
| GenBank Accession Number | BC024043 |
| Gene Symbol | PAN2 |
| Gene ID (NCBI) | 9924 |
| RRID | AB_1851434 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q504Q3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PAN2 antibody 16427-1-AP | Download protocol |
| IHC protocol for PAN2 antibody 16427-1-AP | Download protocol |
| IF protocol for PAN2 antibody 16427-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization.
| ||
Nat Struct Mol Biol An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to mediate mRNA deadenylation and decay. | ||
Neurobiol Dis Distinct Poly(A) nucleases have differential impact on sut-2 dependent tauopathy phenotypes. | ||
Biol Reprod Mobilization of Dormant Cnot7 mRNA Promotes Deadenylation of Maternal Transcripts During Mouse Oocyte Maturation. | ||
PLoS One Anti-PABPC1 Co-Immunoprecipitation for Examining the miRNAs Directly Targeting the 3'-UTR of EED mRNA. | ||
Cells Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN). |
