PD-L1/CD274 Antibody 5 Publications

Mouse Monoclonal| Catalog number: 66248-1-Ig |CloneNo.: 2B11D11

Featured Product KD/KO validated

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Species specificity:
human, mouse, pig, rat

Positive WB detected in:
HepG2 cells, RAW 264.7 cells, HepG2 cells , human skeletal muscle tissue, pig lung tissue, human placenta tissue

Positive IHC detected in:
human tonsillitis tissue, mouse heart tissue, human lung cancer tissue, human placenta tissue, human heart tissue

Positive IF detected in:
HeLa cells

Positive FC detected in:
Raji cells

Recommended dilution:
WB : 1:500-1:2000
IHC : 1:100-1:500
IF : 1:50-1:500

Product Information


Source:
Mouse

Purification method:
Protein G purification

Isotype:
IgG1

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
CD274 molecule

Calculated molecular weight:
290aa,33 kDa

Observed molecular weight:
50 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
CD274

Synonyms
B7 H, B7 H1, B7 homolog 1, B7H1, CD274, CD274 molecule, PD L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, PD-L1, PD-L1/CD274, Programmed death ligand 1
Background

Programmed cell death ligand 1(CD274, or B7-H1, PD-L1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. CD274 is suggested a negative regulator of T and B cell, and play important role in mediating tolerance of lymphocytes to self-antigens. It also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. PD-L1 is a 290 aa transmembrane protein with a calculated molecular weight of 33 kDa, the apparent molecular weight has been reported as 45-70 kDa, suggesting probable glycosylation (PMID: 18760278; 16493058).


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