PF4 Polyclonal antibody
PF4 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, pig
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Human peripheral blood platelets tissue, mouse spleen tissue |
Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
21157-1-AP targets PF4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, pig |
Cited Reactivity | human, mouse |
Immunogen | PF4 fusion protein Ag12345 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | platelet factor 4 |
Synonyms | C X C motif chemokine 4, CXCL4, Iroplact, Oncostatin A, PF 4, PF4, platelet factor 4, SCYB4 |
Calculated Molecular Weight | 101 aa, 11 kDa |
Observed Molecular Weight | 10 kDa |
GenBank Accession Number | BC093965 |
Gene Symbol | PF4 |
Gene ID (NCBI) | 5196 |
RRID | AB_2878819 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P02776 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PF4 antibody 21157-1-AP | Download protocol |
IHC protocol for PF4 antibody 21157-1-AP | Download protocol |
IF protocol for PF4 antibody 21157-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Biosci Neutrophil extracellular traps promote thrombogenicity in cerebral venous sinus thrombosis. | ||
J Vasc Access Functions for platelet factor 4 (PF4/CXCL4) and its receptors in fibroblast-myofibroblast transition and fibrotic failure of arteriovenous fistulas (AVFs) |