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PLSCR3 Polyclonal antibody

PLSCR3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, rat and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 28028-1-AP

Print datasheet

Synonyms

phospholipid scramblase 3, PL scramblase 3, PLSCR3

验证数据展示

  • WB analysis using 28028-1-AP

    WB analysis using 28028-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28028-1-AP (PLSCR3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 28028-1-AP

    IHC staining of human liver cancer using 28028-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28028-1-AP (PLSCR3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 28028-1-AP

    WB analysis using 28028-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28028-1-AP (PLSCR3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse kidney tissue, A431 cells, mouse liver tissue, mouse lung tissue, rat kidney tissue, rat liver tissue, rat lung tissue, BxPC-3 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28028-1-AP targets PLSCR3 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited Applications WB
Tested Reactivity Human, Mouse, rat
Cited Reactivitymouse
Immunogen PLSCR3 fusion protein Ag27655 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipid scramblase 3
Synonyms phospholipid scramblase 3, PL scramblase 3, PLSCR3
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC011735
Gene Symbol PLSCR3
Gene ID (NCBI) 57048
RRIDAB_2881040
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRY6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLSCR3, also named as PLS3, belongs to the phospholipid scramblase family. It may mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. PLSCR3 may play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. It is a target of protein kinase C-δ (PKC-δ), resides in mitochondria and plays pivotal roles in regulating apoptotic response. Phosphorylation of PLSCR3 by PKC-δ is involved in the hyperthermia-induced apoptotic signal transduction pathway in Tca8113 cells, and that Hsp27 blocks this pathway to suppress hyperthermia-induced apoptosis. (PMID:20569108)

实验方案

Product Specific Protocols
WB protocol for PLSCR3 antibody 28028-1-APDownload protocol
IHC protocol for PLSCR3 antibody 28028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Mol Neurosci

Cardiolipin externalization mediates prion protein (PrP) peptide 106-126-associated mitophagy and mitochondrial dysfunction

Authors - Dongming Yang
View Article