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PML Polyclonal antibody

PML Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 21041-1-AP

Print datasheet

Synonyms

MYL, PML, PP8675, promyelocytic leukemia, RING finger protein 71, RNF71, TRIM19

验证数据展示

  • WB analysis of HeLa using 21041-1-AP

    WB analysis of HeLa using 21041-1-AP

    WB result of PML antibody (21041-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PML transfected HeLa cells.

  • WB analysis of HEK-293 using 21041-1-AP

    WB analysis of HEK-293 using 21041-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21041-1-AP (PML Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 21041-1-AP

    IP experiment of HEK-293 using 21041-1-AP

    IP result of anti-PML (IP:21041-1-AP, 4ug; Detection:21041-1-AP 1:500) with HEK-293 cells lysate 3200ug.

  • IHC staining of human liver using 21041-1-AP

    IHC staining of human liver using 21041-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21041-1-AP (PML Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with PML antibody included
  • IHC staining of human liver using 21041-1-AP

    IHC staining of human liver using 21041-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21041-1-AP (PML Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with PML antibody included
  • IF Staining of HeLa using 21041-1-AP

    IF Staining of HeLa using 21041-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PML antibody (21041-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IF Staining of HeLa using 21041-1-AP

    IF Staining of HeLa using 21041-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PML antibody (21041-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PML Ready-To-Use IHC Kit. PML primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21041-1-AP targets PML in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen PML fusion protein Ag15377 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name promyelocytic leukemia
Synonyms MYL, PML, PP8675, promyelocytic leukemia, RING finger protein 71, RNF71, TRIM19
Calculated Molecular Weight 882 aa, 98 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC020994
Gene Symbol PML
Gene ID (NCBI) 5371
RRIDAB_2878799
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29590
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PML, also named as MYL, RNF71 and TRIM19, is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. It functions as tumor suppressor. PML is required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. It plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. PML plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. It regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation. A large number of alternative spliced transcripts are synthesized from the PML gene, resulting in a variety of PML proteins ranging in molecular weight from 48-97 kDa (PMID:11704850). And it can be detected as 70-130 kDa or larger due to the modification (especially SUMO) (PMID: 16778193, 22438555).

实验方案

Product Specific Protocols
WB protocol for PML antibody 21041-1-APDownload protocol
IHC protocol for PML antibody 21041-1-APDownload protocol
IF protocol for PML antibody 21041-1-APDownload protocol
IP protocol for PML antibody 21041-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nat Commun

BioE3 identifies specific substrates of ubiquitin E3 ligases

Authors - Orhi Barroso-Gomila
View Article
humanWB,IF

Nat Commun

Identification of proximal SUMO-dependent interactors using SUMO-ID.

Authors - Orhi Barroso-Gomila
View Article
humanWB

Nucleic Acids Res

mRNAs are sorted for export or degradation before passing through nuclear speckles.

Authors - Jing Fan
View Article
humanIHC,IF

Theranostics

Autophagic degradation of PML promotes susceptibility to HSV-1 by stress-induced corticosterone.

Authors - Wen Li
View Article
humanWB

Front Oncol

Identification of a Two-Gene (PML-EPB41) Signature With Independent Prognostic Value in Osteosarcoma.

Authors - Shengye Liu
View Article
humanWB,IF

J Ginseng Res

20(S)-ginsenoside Rh2 induces caspase-dependent promyelocytic leukemia-retinoic acid receptor A degradation in NB4 cells via Akt/Bax/caspase9 and TNF-α/caspase8 signaling cascades.

Authors - Sirui Zhu
View Article