PPP1CB-Specific Polyclonal antibody
PPP1CB-Specific Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF, IHC, IP, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | human brain tissue, NIH/3T3 |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | NIH/3T3 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10140-2-AP targets PPP1CB-Specific in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF, IHC, IP, WB,ELISA |
Cited Applications | IHC, IP, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, rat |
Immunogen | PPP1CB-Specific fusion protein Ag0183 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | protein phosphatase 1, catalytic subunit, beta isoform |
Synonyms | PP1 Beta, PP 1B, PPP1CB, PPP1CD |
Calculated Molecular Weight | 37 kDa |
Observed Molecular Weight | 37 kDa |
GenBank Accession Number | BC002697 |
Gene Symbol | PPP1CB |
Gene ID (NCBI) | 5500 |
RRID | AB_2168272 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P62140 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PPP1CB-Specific antibody 10140-2-AP | Download protocol |
IHC protocol for PPP1CB-Specific antibody 10140-2-AP | Download protocol |
IF protocol for PPP1CB-Specific antibody 10140-2-AP | Download protocol |
IP protocol for PPP1CB-Specific antibody 10140-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Am J Physiol Cell Physiol PP1γ functionally augments the alternative splicing of CaMKIIδ through interacting with ASF. | ||
Melanoma Res Differential expression patterns of capping protein, protein phosphatase 1, and casein kinase 1 may serve as diagnostic markers for malignant melanoma. | ||
Clin Exp Pharmacol Physiol Rescue of cardiac failing and remodeling by inhibition of PP1γ is associated with suppression of the ASF-mediated splicing of CaMKIIδ |