PPP1CB-Specific Polyclonal antibody

PPP1CB-Specific Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 10140-2-AP

Print datasheet

Synonyms

PP1 Beta, PP 1B, PPP1CB, PPP1CD



经过测试的应用

Positive WB detected inhuman brain tissue, NIH/3T3
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy PPP1CB Ready-To-Use IHC Kit. PPP1CB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10140-2-AP targets PPP1CB-Specific in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB,ELISA
Cited Applications IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Immunogen PPP1CB-Specific fusion protein Ag0183 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1, catalytic subunit, beta isoform
Synonyms PP1 Beta, PP 1B, PPP1CB, PPP1CD
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC002697
Gene Symbol PPP1CB
Gene ID (NCBI) 5500
RRIDAB_2168272
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62140
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein phosphatase 1 (PP1) is the main eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular processes including protein-protein interactions, gene transcription, cell-cycle progression and apoptosis. It is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. The catalytic subunit of PP1 (PP1c) can interact with amount of regulatory subunits in vivo that target it to particular subcellular locations and regulate its activity towards specific substrates. PPP1CB migrated as a 37kd protein on SDS page. This antibody is specific to PPP1CB.

实验方案

Product Specific Protocols
WB protocol for PPP1CB-Specific antibody 10140-2-APDownload protocol
IHC protocol for PPP1CB-Specific antibody 10140-2-APDownload protocol
IF protocol for PPP1CB-Specific antibody 10140-2-APDownload protocol
IP protocol for PPP1CB-Specific antibody 10140-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Am J Physiol Cell Physiol

PP1γ functionally augments the alternative splicing of CaMKIIδ through interacting with ASF.

Authors - Chao Huang
humanWB,IHC

Melanoma Res

Differential expression patterns of capping protein, protein phosphatase 1, and casein kinase 1 may serve as diagnostic markers for malignant melanoma.

Authors - Sun Daxin D
ratWB, IP

Clin Exp Pharmacol Physiol

Rescue of cardiac failing and remodeling by inhibition of PP1γ is associated with suppression of the ASF-mediated splicing of CaMKIIδ

Authors - Ru-Jia Liao