RIPK1-Specific Polyclonal antibody

RIPK1-Specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (3)

Applications

IHC, WB, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 17519-1-AP

Print datasheet

Synonyms

Cell death protein RIP, Receptor interacting protein 1, RIP, RIP 1, RIP1, RIPK1, RIPK1-Specific



经过测试的应用

Positive WB detected inmouse heart tissue, rat liver tissue, rat heart tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17519-1-AP targets RIPK1-Specific in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited Applications CoIP, IF, IHC, IP, WB
Tested Reactivity mouse, rat
Cited Reactivityhuman, mouse, rat, yeast, pig
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name receptor (TNFRSF)-interacting serine-threonine kinase 1
Synonyms Cell death protein RIP, Receptor interacting protein 1, RIP, RIP 1, RIP1, RIPK1, RIPK1-Specific
Calculated Molecular Weight 76 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberNM_003804
Gene Symbol RIPK1
Gene ID (NCBI) 8737
RRIDAB_10642833
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13546
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis, and necroptosis and belongs to a novel class of kinases that function in cell survival and cell death mechanisms(PMID:22685397 ). It has 2 isoforms produced by alternative splicing. It also can exist as a protein with a lower molecular weight of about 25 kDa and 45 kDa in HAEC and HUVEC(PMID:22685397).

实验方案

Product Specific Protocols
WB protocol for RIPK1-Specific antibody 17519-1-APDownload protocol
IHC protocol for RIPK1-Specific antibody 17519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Acta Pharm Sin B

A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein.

Authors - Ziqiao Yuan
mouseWB

Circ Res

AirnRegulates Igf2bp2 Translation in Cardiomyocytes.

Authors - Mohammed R Hosen
mouseWB,IP

Nat Commun

Regulation of RIPK1 activation by TAK1-mediated phosphorylation dictates apoptosis and necroptosis.

Authors - Jiefei Geng
humanIF

Cancer Res

Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.

Authors - Bingteng Xie

PLoS Biol

The Machado-Joseph Disease Deubiquitinase Ataxin-3 Regulates the Stability and Apoptotic Function of p53.

Authors - Hongmei Liu
humanWB

Free Radic Biol Med

TRAF2 inhibits senescence in hepatocellular carcinoma cells via regulating the ROMO1/ NAD+/SIRT3/SOD2 axis

Authors - Jiping Yao