• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SH3D19 Polyclonal antibody

SH3D19 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 21499-1-AP

Print datasheet

Synonyms

ADAM binding protein Eve 1, EBP, EEN binding protein, EVE1, Kryn, SH3 domain containing 19, SH3D19, SH3P19

验证数据展示

  • WB analysis of HT-1080 using 21499-1-AP

    WB analysis of HT-1080 using 21499-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 21499-1-AP (SH3D19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 21499-1-AP

    IHC staining of human heart using 21499-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 21499-1-AP

    IHC staining of human heart using 21499-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 21499-1-AP

    IF Staining of HepG2 using 21499-1-AP

    Immunofluorescent analysis of HepG2 cells, using SH3D19 antibody 21499-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inHT-1080 cells, Y79 cells
Positive IHC detected inhuman heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21499-1-AP is SH3D19 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen SH3D19 fusion protein Ag15915 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SH3 domain containing 19
Synonyms ADAM binding protein Eve 1, EBP, EEN binding protein, EVE1, Kryn, SH3 domain containing 19, SH3D19, SH3P19
Calculated Molecular Weight 790 aa, 87 kDa
Observed Molecular Weight 45-47 kDa
GenBank Accession NumberBC108890
Gene Symbol SH3D19
Gene ID (NCBI) 152503
RRIDAB_10733218
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5HYK7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SH3D19 antibody 21499-1-APDownload protocol
IHC protocol for SH3D19 antibody 21499-1-APDownload protocol
IF protocol for SH3D19 antibody 21499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols