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SIN3A Polyclonal antibody

SIN3A Polyclonal Antibody for IF/ICC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14638-1-AP

Print datasheet

Synonyms

KIAA0700, SIN3A

验证数据展示

  • WB analysis of Jurkat using 14638-1-AP

    WB analysis of Jurkat using 14638-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14638-1-AP (SIN3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 14638-1-AP

    IF Staining of HepG2 using 14638-1-AP

    Immunofluorescent analysis of HepG2 cells, using SIN3A antibody 14638-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of Jurkat using 14638-1-AP

    IP experiment of Jurkat using 14638-1-AP

    IP result of anti-SIN3A (IP:14638-1-AP, 4ug; Detection:14638-1-AP 1:500) with Jurkat cells lysate 3600ug.

经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, K-562 cells
Positive IP detected inJurkat cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14638-1-AP targets SIN3A in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB,ELISA
Cited Applications CoIP, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen SIN3A fusion protein Ag6219 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SIN3 homolog A, transcription regulator (yeast)
Synonyms KIAA0700, SIN3A
Calculated Molecular Weight 145 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC066364
Gene Symbol SIN3A
Gene ID (NCBI) 25942
RRIDAB_2301741
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96ST3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SIN3A antibody 14638-1-APDownload protocol
IF protocol for SIN3A antibody 14638-1-APDownload protocol
IP protocol for SIN3A antibody 14638-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Theranostics

SLC14A1 prevents oncometabolite accumulation and recruits HDAC1 to transrepress oncometabolite genes in urothelial carcinoma.

Authors - Ti-Chun Chan
View Article
humanWB,CoIP

Int J Biol Sci

TET1s deficiency exacerbates oscillatory shear flow-induced atherosclerosis.

Authors - Kai Qu
View Article

Cell Rep

ZNF451 favors triple-negative breast cancer progression by enhancing SLUG-mediated CCL5 transcriptional expression

Authors - Yu Zhang
View Article
mouseWB,IP

Nat Commun

Glucocorticoids increase adiposity by stimulating Krüppel-like factor 9 expression in macrophages

Authors - Yinliang Zhang
View Article