- Featured Product
- KD/KO Validated
SIX1 Polyclonal antibody
SIX1 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
IF, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A2780 cells, HEK-293 cells, HepG2 cells, L02 cells, PC-3 cells, SKOV-3 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IF detected in | epithelial tumor cells |
Planning an IHC experiment? We recommend our IHCeasy SIX1 Ready-To-Use IHC Kit. SIX1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10709-1-AP targets SIX1 in WB,IP,IF,ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IF, IP, WB, ELISA |
| Cited Applications | WB,IHC,IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | SIX1 fusion protein Ag1044 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | SIX homeobox 1 |
| Synonyms | BOS3, DFNA23, Homeobox protein SIX1, Sine oculis homeobox homolog 1, SIX homeobox 1, SIX1, TIP39 |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 32-35 kDa |
| GenBank Accession Number | BC008874 |
| Gene Symbol | SIX1 |
| Gene ID (NCBI) | 6495 |
| RRID | AB_2189077 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15475 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SIX1 belongs to SIX family that contains a divergent DNA-binding homeodomain and an upstream SIX domain, which may participate both in determining DNA-binding specificity and in mediating protein-protein interactions. It may be involved in limb tendon and ligament development, and specifically expressed in skeletal muscle. SIX1 plays a essential role in the development of numerous organs and shows littlt to no expression in most non-neoplastic adult tissue, yet is overexpression in a number of meoplasms where it increases cell proliferation and survival. The calcualted molecular weight of SIX1 is 32kDa, but modified SIX1 is about 35 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SIX1 antibody 10709-1-AP | Download protocol |
| IP protocol for SIX1 antibody 10709-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.
| ||
Nat Commun Muscle progenitor specification and myogenic differentiation are associated with changes in chromatin topology. | ||
Theranostics O-GlcNAcylation of SIX1 enhances its stability and promotes Hepatocellular Carcinoma Proliferation.
| ||
Oncogene The homeoprotein SIX1 controls cellular senescence through the regulation of p16INK4A and differentiation-related genes. | ||
Mol Ther Oncolytics miR-489-3p/SIX1 Axis Regulates Melanoma Proliferation and Glycolytic Potential.
|
