• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CHT1 Polyclonal antibody

CHT1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 21848-1-AP

Print datasheet

Synonyms

CHT, CHT1, hCHT, SLC5A7

验证数据展示

  • WB analysis of mouse brain using 21848-1-AP

    WB analysis of mouse brain using 21848-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21848-1-AP (CHT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 21848-1-AP

    IHC staining of rat brain using 21848-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21848-1-AP (CHT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 21848-1-AP

    IHC staining of human brain using 21848-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain slide using 21848-1-AP (CHT1 antibody at dilution of 1:50.

  • IHC staining of human brain using 21848-1-AP

    IHC staining of human brain using 21848-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain slide using 21848-1-AP (CHT1 antibody at dilution of 1:50.

  • IHC staining of rat brain using 21848-1-AP

    IHC staining of rat brain using 21848-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21848-1-AP (CHT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse heart using 21848-1-AP

    IP experiment of mouse heart using 21848-1-AP

    IP result of anti-CHT1 (IP:21848-1-AP, 4ug; Detection:21848-1-AP 1:500) with mouse heart tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21848-1-AP is CHT1 Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen CHT1 fusion protein Ag16136 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 5 (choline transporter), member 7
Synonyms CHT, CHT1, hCHT, SLC5A7
Calculated Molecular Weight 580 aa, 63 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC111525
Gene Symbol SLC5A7
Gene ID (NCBI) 60482
RRIDAB_2878925
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZV3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHT1 antibody 21848-1-APDownload protocol
IHC protocol for CHT1 antibody 21848-1-APDownload protocol
IP protocol for CHT1 antibody 21848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Mol Metab

Activation of a non-neuronal cholinergic system in visceral white adipose tissue of obese mice and humans

Authors - Ilenia Severi
View Article