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- KD/KO Validated
SMAD4 Polyclonal antibody
SMAD4 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (5)
Applications
IHC, IP, WB, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, HEK-293 cells, NIH/3T3 cells, mouse liver tissue, HCT 116 cells, HEK-293T cells, Neuro-2a cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human placenta tissue, human testis tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy SMAD4 Ready-To-Use IHC Kit. SMAD4 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10231-1-AP targets SMAD4 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IHC, IP, WB, ELISA |
| Cited Applications | ChIP, CoIP, IF, IHC, IP, WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, goat, hamster, pig, Bovine |
| Immunogen | SMAD4 fusion protein Ag0299 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | SMAD family member 4 |
| Synonyms | DPC4, hSMAD4, JIP, MAD homolog 4, MADH4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD4 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 63-70 kDa |
| GenBank Accession Number | BC002379 |
| Gene Symbol | SMAD4 |
| Gene ID (NCBI) | 4089 |
| RRID | AB_2193323 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13485 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members . Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF -mediated phosphorylation of other Smad family members. This antibody is a rabbit polyclonal antibody raised against an internal region of human SMAD4.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SMAD4 antibody 10231-1-AP | Download protocol |
| IHC protocol for SMAD4 antibody 10231-1-AP | Download protocol |
| IP protocol for SMAD4 antibody 10231-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics | ||
Transl Res Deubiquitinase USP13 promotes extracellular matrix expression by stabilizing Smad4 in lung fibroblast cells.
| ||
Acta Pharmacol Sin Role of thrombospondin-1 in high-salt-induced mesenteric artery endothelial impairment in rats
| ||
Stem Cell Reports Gene Correction Reverses Ciliopathy and Photoreceptor Loss in iPSC-Derived Retinal Organoids from Retinitis Pigmentosa Patients. | ||
Oxid Med Cell Longev Smad7 Is Highly Expressed in Human Degenerative Discs and Participates in IL-1β-Induced Apoptosis of Rat AF Cells via the Mitochondria Pathway. |
