SNAPIN Polyclonal antibody

SNAPIN Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 10055-1-AP

Print datasheet

Synonyms

SNAP associated protein, SNAP25BP, SNAPAP, SNAPIN



经过测试的应用

Positive WB detected inA375 cells, HEK-293 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman testis tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10055-1-AP targets SNAPIN in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen SNAPIN fusion protein Ag0101 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SNAP-associated protein
Synonyms SNAP associated protein, SNAP25BP, SNAPAP, SNAPIN
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 15-18 kDa
GenBank Accession NumberBC000761
Gene Symbol SNAPIN
Gene ID (NCBI) 23557
RRIDAB_2192656
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95295
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Snapin, a protein of relative molecular weight of 15kd, is implicated in neurotransmission by binding to SNAP-25. Snapin was enriched in neurons and exclusively located on synaptic vesicle membrane, which may be a PKA target for modulating transmitter release through the cAMP-dependent signal-transduction pathway. This anitbody can recognize the 15-18kd(Monomer) and 30-36kd(Dimer) forms of SNAPIN.

实验方案

Product Specific Protocols
WB protocol for SNAPIN antibody 10055-1-APDownload protocol
IHC protocol for SNAPIN antibody 10055-1-APDownload protocol
IF protocol for SNAPIN antibody 10055-1-APDownload protocol
IP protocol for SNAPIN antibody 10055-1-APDownload protocol
FC protocol for SNAPIN antibody 10055-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

J Thromb Haemost

Dysregulation of PLDN (pallidin) is a mechanism for platelet dense granule deficiency in RUNX1 haplodeficiency.

Authors - G F Mao
humanWB

Am J Hum Genet

A BLOC-1 mutation screen reveals that PLDN is mutated in Hermansky-Pudlak Syndrome type 9.

Authors - Cullinane Andrew R AR
mouseWB

Autophagy

Carbon nanotubes, but not spherical nanoparticles, block autophagy by a shape-related targeting of lysosomes in murine macrophages.

Authors - Vanessa Cohignac
humanWB

Autophagy

HIV-1 Vpr protein impairs lysosome clearance causing SNCA/alpha-synuclein accumulation in neurons.

Authors - Maryline Santerre
humanWB,IF

Autophagy

SNAPIN is critical for lysosomal acidification and autophagosome maturation in macrophages.

Authors - Bo Shi
humanWB

J Cell Biol

LAMTOR/Ragulator is a negative regulator of Arl8b- and BORC-dependent late endosomal positioning.

Authors - Przemyslaw A Filipek