• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SP100 Polyclonal antibody

SP100 Polyclonal Antibody for WB,IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB,IHC,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11377-1-AP

Print datasheet

Synonyms

Lysp100b, Nuclear autoantigen Sp 100, SP100, SP100 nuclear antigen, Speckled 100 kDa

验证数据展示

  • WB analysis of Jurkat using 11377-1-AP

    WB analysis of Jurkat using 11377-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11377-1-AP (SP100 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 11377-1-AP

    IHC staining of human ovary tumor using 11377-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11377-1-AP (SP100 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with SP100 antibody included
  • IHC staining of human ovary tumor using 11377-1-AP

    IHC staining of human ovary tumor using 11377-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11377-1-AP (SP100 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with SP100 antibody included

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SP100 Ready-To-Use IHC Kit. SP100 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11377-1-AP targets SP100 in WB,IF,IHC,ELISA applications and shows reactivity with human samples.

Tested Applications WB,IHC,ELISA
Cited ApplicationsWB,IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen SP100 fusion protein Ag1939 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SP100 nuclear antigen
Synonyms Lysp100b, Nuclear autoantigen Sp 100, SP100, SP100 nuclear antigen, Speckled 100 kDa
Calculated Molecular Weight 879 aa, 100 kDa
Observed Molecular Weight 70-100 kDa, 110-130 kDa
GenBank Accession NumberBC011562
Gene Symbol SP100
Gene ID (NCBI) 6672
RRIDAB_2194302
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23497
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SP100 nuclear antigen, belongs to the nuclear bodies(NBs), has a role in the control of gene expression. It contains a HSR domain, which is important for the nuclear body targeting as well as for the dimerization, and one PxVxL motif, which if required for interaction with chromoshadow domains. Sp100 is an acidic protein that exerts a highly aberrant electrophoretic mobility in SDS-polyacrylamide gel electrophoresis.

实验方案

Product Specific Protocols
WB protocol for SP100 antibody 11377-1-APDownload protocol
IHC protocol for SP100 antibody 11377-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS Pathog

Kaposi's Sarcoma Associated Herpesvirus Tegument Protein ORF75 Is Essential for Viral Lytic Replication and Plays a Critical Role in the Antagonization of ND10-Instituted Intrinsic Immunity.

Authors - Florian Full
View Article

J Virol

THE VIRAL FGARAT HOMOLOG ORF75 OF RHESUS MONKEY RHADINOVIRUS EFFECTS PROTEASOMAL DEGRADATION OF THE ND10 COMPONENTS SP100 AND PML.

Authors - Alexander S Hahn
View Article
human

MBio

Role for a Filamentous Nuclear Assembly of IFI16, DNA, and Host Factors in Restriction of Herpesviral Infection.

Authors - Philipp E Merkl
View Article