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  • Featured Product
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SRF Monoclonal antibody

SRF Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G8F4

Cat No : 66742-1-Ig

Print datasheet

Synonyms

MCM1, Serum response factor, SRF

验证数据展示

  • WB analysis using 66742-1-Ig

    WB analysis using 66742-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 66742-1-Ig

    WB analysis of MCF-7 using 66742-1-Ig

    WB result of SRF antibody (66742-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected MCF-7 cells.

  • IF Staining of HepG2 using 66742-1-Ig

    IF Staining of HepG2 using 66742-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SRF antibody (66742-1-Ig, Clone: 1G8F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, RAW 264.7 cells, MCF-7 cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SRF Ready-To-Use IHC Kit. SRF primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66742-1-Ig targets SRF in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Cited Reactivitymouse
Immunogen SRF fusion protein Ag10386 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name serum response factor (c-fos serum response element-binding transcription factor)
Synonyms MCM1, Serum response factor, SRF
Calculated Molecular Weight 508 aa, 52 kDa
GenBank Accession NumberBC048211
Gene Symbol SRF
Gene ID (NCBI) 6722
RRIDAB_2882090
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11831
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).

实验方案

Product Specific Protocols
WB protocol for SRF antibody 66742-1-IgDownload protocol
IF protocol for SRF antibody 66742-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Ther Nucleic Acids

Diverging targets mediate the pathological roleof miR-199a-5p and miR-199a-3p by promoting cardiac hypertrophy and fibrosis

Authors - Ni Zeng
View Article