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STAT1 Polyclonal antibody

STAT1 Polyclonal Antibody for FC (Intra), IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

FC (Intra), IF, IP, WB, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 10144-2-AP

Print datasheet

Synonyms

ISGF 3, STAT1, STAT91



经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, A549 cells, K-562 cells, PC-3 cells
Positive IP detected inPC-3 cells
Positive IF detected inPC-3 cells
Positive FC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10144-2-AP targets STAT1 in WB, IP, IHC, IF, FC (Intra), CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Applications FC (Intra), IF, IP, WB, ELISA
Cited ApplicationsChIP, CoIP, IF, IHC, IP, WB
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen STAT1 fusion protein Ag0199 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name signal transducer and activator of transcription 1, 91kDa
Synonyms ISGF 3, STAT1, STAT91
Calculated Molecular Weight 83 kDa
Observed Molecular Weight83-87 kDa
GenBank Accession NumberBC002704
Gene Symbol STAT1
Gene ID (NCBI) 6772
RRIDAB_2286875
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42224
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

What is the molecular weight of STAT1? Are there any isoforms of STAT1? Is STAT1 post-translationally modified?

The molecular weight of STAT1 is 91 kDa for full-length STAT1α and 84 kDa for STAT1β, which is devoid of the C-terminal activation domain (PMID: 1502203). Phosphorylation of STAT1 regulates STAT1 activity. Phosphorylation of Tyr701 by Janus kinases (JAKs) triggers STAT1 homo- and hetero-dimerization with other STAT family members and also causes nuclear translocation, while phosphorylation of Ser727 is needed for full activation (PMID: 10851062). STAT1β lacks Ser727 and has therefore been considered to be inactive or acting in a dominant negative manner to STAT1α, although it has been further shown to be capable of transcription activation in vivo (PMID: 24710278).

 What is the subcellular localization of STAT1?

STAT1 is present in the cytoplasm and in the nucleus. Translocation of STAT1 from the cytoplasm to the nucleus is required for its regulation of gene expression and is dependent on phosphorylation (see above). However, low levels of unphosphorylated STAT1, often referred to as U-STAT1, can also be found in the nucleus, where U-STAT1 regulates the stability of heterochromatin by binding to chromatin in monomeric or dimeric states (PMID: 18840523).

 What is the tissue expression pattern of STAT1?

STAT1 is ubiquitously expressed.


实验方案

Product Specific Protocols
WB protocol for STAT1 antibody 10144-2-APDownload protocol
IHC protocol for STAT1 antibody 10144-2-APDownload protocol
IF protocol for STAT1 antibody 10144-2-APDownload protocol
IP protocol for STAT1 antibody 10144-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell

Opioid-induced fragile-like regulatory T cells contribute to withdrawal

Authors - Yongsheng Zhu
human,mouseWB,IF

Nat Commun

ID1 expressing macrophages support cancer cell stemness and limit CD8+ T cell infiltration in colorectal cancer

Authors - Shuang Shang
humanWB

Sci Transl Med

TRIB3 reduces CD8+ T cell infiltration and induces immune evasion by repressing the STAT1-CXCL10 axis in colorectal cancer.

Authors - Shuang Shang
mouseWB

Small

Cell-Free Extracts from Human Fat Tissue with a Hyaluronan-Based Hydrogel Attenuate Inflammation in a Spinal Cord Injury Model through M2 Microglia/Microphage Polarization.

Authors - Guang-Yu Xu
mouseWB,IF

Nat Commun

Osteoblasts secrete Cxcl9 to regulate angiogenesis in bone.

Authors - Bin Huang
humanWB

Redox Biol

Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer.

Authors - Shumin Ouyang