STAT4 Polyclonal antibody

STAT4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 51070-2-AP

Print datasheet

Synonyms

SLEB11, STAT4



经过测试的应用

Positive WB detected inA549 cells, HeLa cells, mouse testis tissue, rat testis tissue
Planning an IHC experiment? We recommend our IHCeasy STAT4 Ready-To-Use IHC Kit. STAT4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

51070-2-AP targets STAT4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA
Cited Applications WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name signal transducer and activator of transcription 4
Synonyms SLEB11, STAT4
Calculated Molecular Weight 748 aa, 86 kDa
Observed Molecular Weight 86 kDa
GenBank Accession NumberBC031212
Gene Symbol STAT4
Gene ID (NCBI) 6775
RRIDAB_2286750
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14765
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The JAK/STAT pathway is an extensive signaling pathway downstream of cytokine receptors. STATs are cytosolic proteins with a common structure consisting of an N-terminal oligomerization domain, which favors formation of STAT dimers, followed by a DNA-binding domain and a C-terminal SRC homology-2 (SH2) domain, which is involved in association between STATs and receptors[PMID:22383755]. Signal Transducer and Activator of Transcription 4 (STAT4) is a transcription factor that is activated by IL-12 signaling and promotes Th1-cell differentiation and IFN-γ production [PMID:21998209].

实验方案

Product Specific Protocols
WB protocol for STAT4 antibody 51070-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Immunol

Malat1 Suppresses Immunity to Infection through Promoting Expression of Maf and IL-10 in Th Cells.

Authors - James P Hewitson
humanWB

Food Chem

Cytotoxic withanolides from the stems and leaves of Physalis ixocarpa

Authors - Ke Xiang