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STX3 Monoclonal antibody

STX3 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B9C7

Cat No : 66760-1-Ig

Print datasheet

Synonyms

STX3, STX3A, syntaxin 3

验证数据展示

  • WB analysis using 66760-1-Ig

    WB analysis using 66760-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 66760-1-Ig

    IHC staining of human kidney using 66760-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66760-1-Ig (STX3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 66760-1-Ig

    IHC staining of human kidney using 66760-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66760-1-Ig (STX3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HSC-T6 cells, rat brain tissue, SH-SY5Y cells, NIH/3T3 cell, RAW 264.7 cell, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66760-1-Ig targets STX3 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, rat, mouse
Immunogen STX3 fusion protein Ag7805 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name syntaxin 3
Synonyms STX3, STX3A, syntaxin 3
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC007405
Gene Symbol STX3
Gene ID (NCBI) 6809
RRIDAB_2882106
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13277
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntaxin-3 (also known as STX3) is a member of the syntaxin. Syntaxin-3 is a significant apical targeting protein in the epithelial membrane and in exocytosis process; it also acts as a vesicle transporter by cellular receptor in neutrophils, which is crucial for protein trafficking event (PMID: 25630102). It has been reported that syntaxin-3 has an important role in the growth of neurites, and serves as a direct target for omega-6 arachidonic acid (PMID: 1659826).

实验方案

Product Specific Protocols
WB protocol for STX3 antibody 66760-1-IgDownload protocol
IHC protocol for STX3 antibody 66760-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols