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  • KD/KO Validated

HIC5 Polyclonal antibody

HIC5 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 10565-1-AP

Print datasheet

Synonyms

ARA55, HIC 5, HIC5, TGFB1I1, TSC 5



经过测试的应用

Positive WB detected inHeLa cells, A431 cells, mouse colon tissue, rat colon tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy TGFB1I1/HIC5 Ready-To-Use IHC Kit. TGFB1I1/HIC5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10565-1-AP targets HIC5 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen HIC5 fusion protein Ag0868 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transforming growth factor beta 1 induced transcript 1
Synonyms ARA55, HIC 5, HIC5, TGFB1I1, TSC 5
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48-50 kDa
GenBank Accession NumberBC001830
Gene Symbol TGFB1I1
Gene ID (NCBI) 7041
RRIDAB_2202045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43294
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HIC5 antibody 10565-1-APDownload protocol
IHC protocol for HIC5 antibody 10565-1-APDownload protocol
IF protocol for HIC5 antibody 10565-1-APDownload protocol
IP protocol for HIC5 antibody 10565-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,ratIF,WB,IHC

Acta Pharmacol Sin

GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy

Authors - Ji-Chao Wu
humanWB,IF,IHC

Aging (Albany NY)

Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway.

Authors - Liansheng Sha
  • KD Validated
humanWB

Eur J Pharmacol

Vitamin D3 analogue calcipotriol inhibits the profibrotic effects of transforming growth factor- β1 on pancreatic stellate cells

Authors - Meifang Zheng
humanWB

J Proteome Res

Quantitative proteomics implicates Rictor/mTORC2 in cell adhesion.

Authors - Hao Wang
humanIHC,IF

Cell Death Dis

HIC-5 in cancer-associated fibroblasts contributes to esophageal squamous cell carcinoma progression.

Authors - Xuanling Du
humanIHC

Med Sci Monit

Bioinformatics Profiling and Experimental Validation of 4 Differentially-Expressed LIM Genes in the Course of Colorectal-Adenoma-Carcinoma

Authors - Ying He