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UBC9 Polyclonal antibody

UBC9 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 10224-1-AP

Print datasheet

Synonyms

C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I

验证数据展示

  • WB analysis of mouse spleen using 10224-1-AP

    WB analysis of mouse spleen using 10224-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:50 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 10224-1-AP

    IHC staining of human lung cancer using 10224-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 10224-1-AP (UBC9 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with UBE2I/UBC9 antibody included
  • IHC staining of human lung cancer using 10224-1-AP

    IHC staining of human lung cancer using 10224-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 10224-1-AP (UBC9 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with UBE2I/UBC9 antibody included

经过测试的应用

Positive WB detected inmouse spleen tissue, HEK-293 cells, human testis tissue, Jurkat cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy UBE2I/UBC9 Ready-To-Use IHC Kit. UBE2I/UBC9 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10224-1-AP targets UBC9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen UBC9 fusion protein Ag0252 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
Synonyms C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC000427
Gene Symbol UBE2I
Gene ID (NCBI) 7329
RRIDAB_2210593
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP63279
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).

实验方案

Product Specific Protocols
WB protocol for UBC9 antibody 10224-1-APDownload protocol
IHC protocol for UBC9 antibody 10224-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols