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  • KD/KO Validated

UBE2T/HSPC150 Polyclonal antibody

UBE2T/HSPC150 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10105-2-AP

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Synonyms

HSPC150, PIG50, UBE2T, UBE2T/HSPC150, Ubiquitin carrier protein T, Ubiquitin protein ligase T



经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, Jurkat cells, SKOV-3 cells
Positive IP detected inHeLa cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10105-2-AP targets UBE2T/HSPC150 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IP, WB, ELISA
Cited Applications IF, IHC, IP, WB
Tested Reactivity human
Cited Reactivityhuman
Immunogen UBE2T/HSPC150 fusion protein Ag0153 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2T (putative)
Synonyms HSPC150, PIG50, UBE2T, UBE2T/HSPC150, Ubiquitin carrier protein T, Ubiquitin protein ligase T
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC004152
Gene Symbol UBE2T
Gene ID (NCBI) 29089
RRIDAB_2211478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NPD8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ubiquitin (Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs (TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.

实验方案

Product Specific Protocols
WB protocol for UBE2T/HSPC150 antibody 10105-2-APDownload protocol
IF protocol for UBE2T/HSPC150 antibody 10105-2-APDownload protocol
IP protocol for UBE2T/HSPC150 antibody 10105-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Am J Hum Genet

Mutations in the Gene Encoding the E2 Conjugating Enzyme UBE2T Cause Fanconi Anemia.

Authors - Asuka Hira
humanWB

Cancer Res

Proteasome function is required for DNA damage response and fanconi anemia pathway activation.

Authors - Céline Jacquemont
humanWB

Blood Adv

Amplification and overexpression of E2 ubiquitin conjugase UBE2T promotes homologous recombination in multiple myeloma.

Authors - David A Alagpulinsa
humanWB,IHC

Oncotarget

UBE2T promotes nasopharyngeal carcinoma cell proliferation, invasion, and metastasis by activating the AKT/GSK3β/β-catenin pathway.

Authors - Wei Hu
  • KD Validated
humanWB

Biomed Pharmacother

Affinity-based protein profiling to reveal targets of puerarin involved in its protective effect on cardiomyocytes.

Authors - Shuai Huang

Tumour Biol

Ubiquitin-Conjugating Enzyme E2T is an Independent Prognostic Factor and Promotes Gastric Cancer Progression.

Authors - Hao Yu