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UQCRFS1 Polyclonal antibody

UQCRFS1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 18443-1-AP

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Synonyms

Complex III subunit 5, Complex III subunit IX, Rieske iron sulfur protein, RIP1, RIS1, RISP, UQCR5, UQCRFS1



经过测试的应用

Positive WB detected inMCF-7 cells, mouse heart tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy UQCRFS1 Ready-To-Use IHC Kit. UQCRFS1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18443-1-AP targets UQCRFS1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited Applications IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Immunogen UQCRFS1 fusion protein Ag13315 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1
Synonyms Complex III subunit 5, Complex III subunit IX, Rieske iron sulfur protein, RIP1, RIS1, RISP, UQCR5, UQCRFS1
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC010035
Gene Symbol UQCRFS1
Gene ID (NCBI) 7386
RRIDAB_10699875
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP47985
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ubiquinol cytochrome c reductase UQCRFS1 is a key subunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain. The UQCRFS1 gene encode a 30 kDa protein with a transit peptide and it appears to be involved in development of more aggressive phenotype of breast cancer(PMID:15047214). UQCRFS1 is also named as RISP, complex III subunit 5, cytochrome b-c1 complex subunit 5 and rieske iron-sulfur protein. This antibody is specific to UQCRFS1.

实验方案

Product Specific Protocols
WB protocol for UQCRFS1 antibody 18443-1-APDownload protocol
IHC protocol for UQCRFS1 antibody 18443-1-APDownload protocol
IP protocol for UQCRFS1 antibody 18443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Res

NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.

Authors - Tingting Hou
humanWB

EMBO J

EglN2 associates with the NRF1-PGC1α complex and controls mitochondrial function in breast cancer.

Authors - Jing Zhang
pigWB

J Cachexia Sarcopenia Muscle

MyD88 deficiency ameliorates weight loss caused by intestinal oxidative injury in an autophagy-dependent mechanism.

Authors - Ming Qi
human,mouseWB

Cell Biosci

Hinokitiol-iron complex is a ferroptosis inducer to inhibit triple-negative breast tumor growth

Authors - Hongting Zhao
mouseWB

J Nanobiotechnology

Cur@SF NPs alleviate Friedreich's ataxia in a mouse model through synergistic iron chelation and antioxidation.

Authors - Li Xu
humanWB

Int J Mol Sci

Contemporary Antiretroviral Therapy Dysregulates Iron Transport and Augments Mitochondrial Dysfunction in HIV-Infected Human Microglia and Neural-Lineage Cells

Authors - Harpreet Kaur