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VDAC2 Polyclonal antibody

VDAC2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 55261-1-AP

Print datasheet

Synonyms

hVDAC2, VDAC 2, VDAC2

验证数据展示

  • WB analysis of HepG2 using 55261-1-AP

    WB analysis of HepG2 using 55261-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55261-1-AP (VDAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 55261-1-AP

    IHC staining of human kidney using 55261-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55261-1-AP (VDAC2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 55261-1-AP

    IHC staining of human kidney using 55261-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55261-1-AP (VDAC2 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHepG2 cells, mouse brain tissue, HuH-7 cells
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

55261-1-AP targets VDAC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Cited Applications WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name voltage-dependent anion channel 2
Synonyms hVDAC2, VDAC 2, VDAC2
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 31-33 kDa
GenBank Accession NumberNM_003375
Gene Symbol VDAC2
Gene ID (NCBI) 7417
RRIDAB_10852961
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP45880
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

VDAC2 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. This antibody is specific to VDAC2.

实验方案

Product Specific Protocols
WB protocol for VDAC2 antibody 55261-1-APDownload protocol
IHC protocol for VDAC2 antibody 55261-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Physiol Biochem

Knockout of VDAC1 in H9c2 Cells Promotes Oxidative Stress-Induced Cell Apoptosis through Decreased Mitochondrial Hexokinase II Binding and Enhanced Glycolytic Stress.

Authors - Meiying Yang
View Article