• Featured Product
  • KD/KO Validated

YTHDF2 Polyclonal antibody

YTHDF2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

IF, IP, WB, ELISA and More (6)

Conjugate

Unconjugated

Cat No : 24744-1-AP

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Synonyms

CLL associated antigen KW 14, HGRG8, NY REN 2, YTH domain family protein 2, YTH domain family, member 2, YTHDF2



经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, HeLa cells, MOLT-4 cells, NIH/3T3 cells, C6 cells, Raji cells
Positive IP detected inHeLa cells
Positive IF detected inmouse brain tissue, U2OS cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy YTHDF2 Ready-To-Use IHC Kit. YTHDF2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24744-1-AP targets YTHDF2 in WB, RIP, IP, IHC, IF, FC, CoIP, CLIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IP, WB, ELISA
Cited Applications chIP, CLIP, CoIP, FC, IF, IHC, IP, RIP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, monkey, pig
Immunogen YTHDF2 fusion protein Ag5922 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name YTH domain family, member 2
Synonyms CLL associated antigen KW 14, HGRG8, NY REN 2, YTH domain family protein 2, YTH domain family, member 2, YTHDF2
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC002559
Gene Symbol YTHDF2
Gene ID (NCBI) 51441
RRIDAB_2687435
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5A9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YTHDF2 also named as CLL associated antigen KW 14 or HGRG8 is a 579 amino acid protein, which contains one YTH domain and exists as two alternatively spliced isoforms. YTHDF2 is expressed in in pancreas, testis and placenta. YTHDF2 may play a role in human longevity.

实验方案

Product Specific Protocols
WB protocol for YTHDF2 antibody 24744-1-APDownload protocol
IF protocol for YTHDF2 antibody 24744-1-APDownload protocol
IP protocol for YTHDF2 antibody 24744-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nature

m6A enhances the phase separation potential of mRNA.

Authors - Ryan J Ries
mouseRIP

Nature

m6A RNA methylation regulates the fate of endogenous retroviruses.

Authors - Tomasz Chelmicki
  • KO Validated
mouseWB

Nature

m6A facilitates hippocampus-dependent learning and memory through YTHDF1.

Authors - Hailing Shi
human, mouseWB, IF

Nature

Dynamic m(6)A mRNA methylation directs translational control of heat shock response.

Authors - Jun Zhou
humanWB

Cell

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.

Authors - Peiguo Yang
human,mouseFC

Nat Med

Stem cell architecture drives myelodysplastic syndrome progression and predicts response to venetoclax-based therapy.

Authors - Irene Ganan-Gomez