• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-27 protein

Activity

50-250 ng/mL EC50 in OCI-AML5 cells

Species Reactivity

human

Purity

>95%

Cat No : HZ-1275

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Synonyms

IL 27, IL 27 A, IL 27 subunit alpha, IL 27A, IL27, IL-27, IL27 A, IL27A, IL27p28, IL30, interleukin 27, Interleukin 27 subunit alpha, p28



Technical Specifications

GeneID 246778
Species Human
Expression HEK293
Activity 50-250 ng/mL EC50 in OCI-AML5 cells
Purity >95%
Endotoxin<1 EU/μg
Accession NumberQ8NEV9
Molecular Mass 25 and 30 kDa reduced and non-reduced, heterodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

IL-27 is a heterodimeric cytokine composed of two subunits: IL-27a and IL-27b. IL-27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL-27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL-10 producing type 1 regulatory T cells. IL-27 is a potentially promising cytokine for therapeutic approaches on various human diseases (PMID: 22608254;16081786;16670296;16906167)

Synonyms

IL 27, IL 27 A, IL 27 subunit alpha, IL 27A, IL27, IL-27, IL27 A, IL27A, IL27p28, IL30, interleukin 27, Interleukin 27 subunit alpha, p28

Publications

SpeciesTitle
human

J Exp Med

IL-27 inhibits HIV-1 infection in human macrophages by down-regulating host factor SPTBN1 during monocyte to macrophage differentiation.

Authors - LueDai
human

Stem Cell Res Ther

hPMSCs inhibit the expression of PD-1 in CD4+IL-10+ T cells and mitigate liver damage in a GVHD mouse model by regulating the crosstalk between Nrf2 and NF-κB signaling pathway.

Authors - Aiping Zhang