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- KD/KO Validated
Syntaxin 6 Polyclonal antibody
Syntaxin 6 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Neuro-2a cells, human brain tissue, mouse brain tissue, rat brain tissue, A549 cells, PC-12 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | PC-12 cells, murine BM-DC, murine Spleen-DC |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:350-1:1400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10841-1-AP targets Syntaxin 6 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | FC, IF, WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Syntaxin 6 fusion protein Ag1284 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | syntaxin 6 |
| Synonyms | Cell and organelle markers, Golgi Marker, STX6, syntaxin 6 |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC009944 |
| Gene Symbol | STX6 |
| Gene ID (NCBI) | 10228 |
| RRID | AB_2196506 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43752 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Syntaxin 6 antibody 10841-1-AP | Download protocol |
| IHC protocol for Syntaxin 6 antibody 10841-1-AP | Download protocol |
| IF protocol for Syntaxin 6 antibody 10841-1-AP | Download protocol |
| IP protocol for Syntaxin 6 antibody 10841-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science IRAP identifies an endosomal compartment required for MHC class I cross-presentation. | ||
Cell Rep Med Compromised mitochondrial quality control triggers lipin1-related rhabdomyolysis. | ||
Nat Commun IRAP-dependent endosomal T cell receptor signalling is essential for T cell responses. | ||
Cell Rep Innate Immune Signals Induce Anterograde Endosome Transport Promoting MHC Class I Cross-Presentation. | ||
J Immunol Conventional dendritic cells require IRAP-Rab14 endosomes for efficient cross-presentation. |
