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  • KD/KO Validated

ARL3 Polyclonal antibody

ARL3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10961-1-AP

Print datasheet

Synonyms

ADP ribosylation factor like 3, ARFL3, ARL3



经过测试的应用

Positive WB detected inmouse brain tissue, PC-3 cells, human brain tissue, mouse testis tissue, rat brain tissue, HeLa cells, rat testis tissue
Positive IHC detected inhuman testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells, IMCD3 cells, hTERT-RPE1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10961-1-AP targets ARL3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsIF, IHC, IP, WB
Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse
Immunogen ARL3 fusion protein Ag1406 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ADP-ribosylation factor-like 3
Synonyms ADP ribosylation factor like 3, ARFL3, ARL3
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 20 kDa, 25 kDa
GenBank Accession NumberBC009841
Gene Symbol ARL3
Gene ID (NCBI) 403
RRIDAB_2274220
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36405
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARL3 (ADP-ribosylation factor-like 3) is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. ARL3 is believed to be involved in ciliary and microtubule-dependent processes.

实验方案

Product Specific Protocols
WB protocol for ARL3 antibody 10961-1-APDownload protocol
IHC protocol for ARL3 antibody 10961-1-APDownload protocol
IF protocol for ARL3 antibody 10961-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nat Commun

A stress-induced cilium-to-PML-NB route drives senescence initiation

Authors - Xiaoyu Ma
humanWB,IF

Nat Commun

Spatial cycles mediated by UNC119 solubilisation maintain Src family kinases plasma membrane localisation.

Authors - Antonios D Konitsiotis
mouseWB,IF, IP

Nat Commun

Arl3 and LC8 regulate dissociation of dynactin from dynein.

Authors - Mingyue Jin
  • KD Validated
mouseWB

Nat Commun

Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism.

Authors - Hyunho Kim
  • KD Validated
humanWB

Proc Natl Acad Sci U S A

ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.

Authors - Humbert Melissa C MC
humanIF

Dev Cell

The Ciliary Machinery Is Repurposed for T Cell Immune Synapse Trafficking of LCK.

Authors - Louise A Stephen