• Featured Product
  • KD/KO Validated

Caveolin-1 Polyclonal antibody

Caveolin-1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 16447-1-AP

Print datasheet

Synonyms

BSCL3, CAV, CAV1, Caveolin 1, Caveolin-1, CGL3, MSTP085, VIP21



经过测试的应用

Positive WB detected inA549 cells, HeLa cells, NIH/3T3 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue, mouse brain tissue, human liver tissue, human lung cancer tissue, human lung tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Caveolin-1 Ready-To-Use IHC Kit. Caveolin-1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16447-1-AP targets Caveolin-1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsIF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish, canine, bovine
Immunogen Caveolin-1 fusion protein Ag8049 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name caveolin 1, caveolae protein, 22kDa
Synonyms BSCL3, CAV, CAV1, Caveolin 1, Caveolin-1, CGL3, MSTP085, VIP21
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 20-25 kDa
GenBank Accession NumberBC006432
Gene Symbol CAV1
Gene ID (NCBI) 857
RRIDAB_10732595
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ03135
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in astric Cancer (PMID: 25339030). MW of Caveolin-1 is from 20-25 kDa due to phosphorylation (PMID: 10198051).

实验方案

Product Specific Protocols
WB protocol for Caveolin-1 antibody 16447-1-APDownload protocol
IHC protocol for Caveolin-1 antibody 16447-1-APDownload protocol
IP protocol for Caveolin-1 antibody 16447-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Res

RAB31 marks and controls an ESCRT-independent exosome pathway.

Authors - Denghui Wei
humanWB

Nucleic Acids Res

Nucleosomes enter cells by clathrin- and caveolin-dependent endocytosis.

Authors - Huawei Wang
mouseWB

Dev Cell

Vangl2 limits chaperone-mediated autophagy to balance osteogenic differentiation in mesenchymal stem cells.

Authors - Yan Gong
human,mouseWB,IF

Redox Biol

Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis

Authors - Mengyue You
mouseWB

Biomaterials

Combination antitumor immunotherapy with VEGF and PIGF siRNA via systemic delivery of multi-functionalized nanoparticles to tumor-associated macrophages and breast cancer cells.

Authors - Yudong Song
humanIF

Small

Smart Carbon Nanotubes with Laser-Controlled Behavior in Gene Delivery and Therapy through a Non-Digestive Trafficking Pathway.

Authors - Fenfen Kong