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- KD/KO Validated
RB1CC1 Polyclonal antibody
RB1CC1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IF/ICC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, K-562 cells, Jurkat cells, MCF-7 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, MCF-7 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 750 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17250-1-AP targets RB1CC1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Immunogen | RB1CC1 fusion protein Ag10508 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | RB1-inducible coiled-coil 1 |
| Synonyms | CC1, DRAGOU14, FIP200, KIAA0203, RB1 inducible coiled coil 1, RB1CC1, RBICC |
| Calculated Molecular Weight | 1594 aa, 183 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | BC017556 |
| Gene Symbol | RB1CC1 |
| Gene ID (NCBI) | 9821 |
| RRID | AB_10666428 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TDY2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RB1CC1, also named as RBICC or FIP200, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1. the calculated molecular weight of RB1CC1 is 180 kDa, but the modified RB1CC1 is about 200 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RB1CC1 antibody 17250-1-AP | Download protocol |
| IHC protocol for RB1CC1 antibody 17250-1-AP | Download protocol |
| IF protocol for RB1CC1 antibody 17250-1-AP | Download protocol |
| IP protocol for RB1CC1 antibody 17250-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Immunol Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense. | ||
Cell Res A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.
| ||
Nat Cell Biol The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles.
| ||
Mol Cell TNIP1 inhibits selective autophagy via bipartite interaction with LC3/GABARAP and TAX1BP1 |
