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  • Featured Product
  • KD/KO Validated

PERK/EIF2AK3 Polyclonal antibody

PERK/EIF2AK3 Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20582-1-AP

Print datasheet

Synonyms

EIF2AK3, HRI, HsPEK, Pancreatic eIF2 alpha kinase, PEK, PERK, PERK/EIF2AK3, WRS

验证数据展示

  • WB analysis using 20582-1-AP

    WB analysis using 20582-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20582-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 20582-1-AP

    IHC staining of human pancreas cancer using 20582-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20582-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 20582-1-AP

    IF Staining of HeLa using 20582-1-AP

    Immunofluorescent analysis of HeLa cells using 20582-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 20582-1-AP

    FC experiment of HepG2 using 20582-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PERK/EIF2AK3 (20582-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20582-1-AP targets PERK/EIF2AK3 in WB,IF,FC,IHC,ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB,IF,IHC
Tested Reactivity human
Cited Reactivityhuman, pig, bovine
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 2-alpha kinase 3
Synonyms EIF2AK3, HRI, HsPEK, Pancreatic eIF2 alpha kinase, PEK, PERK, PERK/EIF2AK3, WRS
Calculated Molecular Weight 125 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberNM_004836
Gene Symbol EIF2AK3
Gene ID (NCBI) 9451
RRIDAB_10695760
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZJ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts potentially as a metabolic sensor in the INS-secreting beta-cells to modulate the trafficking and quality control of pro-INS in the ER relative to the physiological demands for circulating INS (PMID:20530744). PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170 kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327). This antibody is specific to PERK.

实验方案

Product Specific Protocols
WB protocol for PERK/EIF2AK3 antibody 20582-1-APDownload protocol
IHC protocol for PERK/EIF2AK3 antibody 20582-1-APDownload protocol
IF protocol for PERK/EIF2AK3 antibody 20582-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Nat Commun

Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy.

Authors - Davy Vanhoutte
View Article
WB

Oxid Med Cell Longev

Curcumin in Combination with Aerobic Exercise Improves Follicular Dysfunction via Inhibition of the Hyperandrogen-Induced IRE1α/XBP1 Endoplasmic Reticulum Stress Pathway in PCOS-Like Rats.

Authors - Yaling Zhang
View Article
humanWB,IHC

Phytother Res

Activation of endoplasmic reticulum stress by harmine suppresses the growth of esophageal squamous cell carcinoma

Authors - Ronghui Fan
View Article
humanWB

Cell Death Discov

Cysteine dioxygenase 1 attenuates the proliferation via inducing oxidative stress and integrated stress response in gastric cancer cells

Authors - Gang Ma
  • KD Validated
View Article
humanWB

Mol Oncol

The synthetic oleanane triterpenoid CDDO-2P-Im binds GRP78/BiP to induce unfolded protein response-mediated apoptosis in myeloma

Authors - George Luo
  • KO Validated
View Article
humanWB

J Med Chem

Cytotoxic BODIPY-Appended Half-Sandwich Iridium(III) Complex Forms Protein Adducts and Induces ER Stress.

Authors - Robin Ramos
View Article