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  • KD/KO Validated

p57Kip2 Polyclonal antibody

p57Kip2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 23317-1-AP

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Synonyms

BWCR, BWS, CDKN1C, KIP2, p57, p57Kip2, WBS



经过测试的应用

Positive WB detected inHeLa cells, SH-SY5Y cells, Jurkat cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23317-1-AP targets p57Kip2 in WB, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited Applications CoIP, IHC, WB
Tested Reactivity human
Cited Reactivityhuman
Immunogen p57Kip2 fusion protein Ag19849 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin-dependent kinase inhibitor 1C (p57, Kip2)
Synonyms BWCR, BWS, CDKN1C, KIP2, p57, p57Kip2, WBS
Calculated Molecular Weight 316 aa, 32 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC067842
Gene Symbol CDKN1C
Gene ID (NCBI) 1028
RRIDAB_2879254
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49918
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDKN1C, also named as p57KIP2 and KIP2, belongs to the CDI family. CDKN1C is a potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. It is a negative regulator of cell proliferation. CDKN1C may play a role in maintenance of the non-proliferative state throughout life.

实验方案

Product Specific Protocols
WB protocol for p57Kip2 antibody 23317-1-APDownload protocol
IHC protocol for p57Kip2 antibody 23317-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Clin Cancer Res

microRNA-221 Enhances MYCN via Targeting Nemo-like Kinase and Functions as an Oncogene Related to Poor Prognosis in Neuroblastoma.

Authors - Xiao-Yan He
humanWB

FASEB J

Downregulation of ALKBH5 rejuvenates aged human mesenchymal stem cells and enhances their therapeutic efficacy in myocardial infarction

Authors - Xiaoyan Gao
humanWB,IHC

Cancer Med

PIK3R3 is upregulated in liver cancer and activates Akt signaling to control cancer growth by regulation of CDKN1C and SMC1A

Authors - Weidong Lin
  • KD Validated
humanWB,CoIP,IHC

Cell Death Dis

The FOXM1/RNF26/p57 axis regulates the cell cycle to promote the aggressiveness of bladder cancer.

Authors - Lu Yi
humanWB

Oncogene

Dual network analysis of transcriptome data for discovery of new therapeutic targets in non-small cell lung cancer

Authors - Yuquan Bai