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KD/KO Validated

Cyclin D1 Polyclonal antibody

Cyclin D1 Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Publications

349

Cat No : 26939-1-AP

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Synonyms

B cell lymphoma 1 protein, CCND1, cyclin D, Cyclin D1, D11S287E, G1/S specific cyclin D1, PRAD1, PRAD1 oncogene, U21B31

验证数据展示

WB analysis using 26939-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26939-1-AP (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 26939-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26939-1-AP (Cyclin D1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 26939-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 26939-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of SH-SY5Y using 26939-1-AP
1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human Cyclin D1 (26939-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
IF Staining of MCF-7 using 26939-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cyclin D1 antibody (26939-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).
IF Staining of NIH/3T3 using 26939-1-AP
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Cyclin D1 antibody (26939-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
WB analysis of HeLa using 26939-1-AP
Non-treated HeLa and thymidine treated HeLa cells were subjected to SDS PAGE followed by western blot with 26939-1-AP (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.

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Cat No. 26939-1-AP

Size

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经过测试的应用

Positive WB detected in	LNCaP cells, MCF-7 cells, C6 cells, NIH/3T3 cells, HeLa cells, Neuro-2a cells, mouse lung tissue
Positive IHC detected in	human ovary tumor tissue, human oesophagus cancer tissue, human lung cancer tissue, mouse spleen tissue, mouse pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	MCF-7 cells, NIH/3T3 cells
Positive FC detected in	SH-SY5Y cells, MCF-7 cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:750-1:3000
Immunofluorescence (IF)	IF : 1:400-1:1600
Flow Cytometry (FC)	FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 337 publications below
IHC	See 25 publications below
IF	See 7 publications below

产品信息

26939-1-AP targets Cyclin D1 in WB,IF,FC,IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	FC, IF/ICC, IHC, WB, ELISA
Cited Applications	WB,IF,IHC
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, goat, sheep, pig, canine, bovine
Immunogen	Cyclin D1 fusion protein Ag25393 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	cyclin D1
Synonyms	B cell lymphoma 1 protein, CCND1, cyclin D, Cyclin D1, D11S287E, G1/S specific cyclin D1, PRAD1, PRAD1 oncogene, U21B31
Calculated Molecular Weight	295 aa, 34 kDa
Observed Molecular Weight	34 kDa
GenBank Accession Number	BC000076
Gene Symbol	CCND1
Gene ID (NCBI)	595
RRID	AB_2880691
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P24385
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.

实验方案

Product Specific Protocols
WB protocol for Cyclin D1 antibody 26939-1-AP	Download protocol
IHC protocol for Cyclin D1 antibody 26939-1-AP	Download protocol
IF protocol for Cyclin D1 antibody 26939-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Mol Cancer LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer. Authors - Xi Jin View Article
human	WB,IHC	Adv Sci (Weinh) Transgelin Promotes Glioblastoma Stem Cell Hypoxic Responses and Maintenance Through p53 Acetylation Authors - Huan Li View Article
human	WB	J Pineal Res Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis. Authors - Li Cui View Article
human	IF	Theranostics Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI Authors - Bojun Li View Article
human	WB	Nat Commun Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma. Authors - Dan Su View Article
human	WB	J Exp Clin Cancer Res ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK Authors - Li Cui View Article

Cyclin D1 Polyclonal antibody

Cyclin D1 Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

验证数据展示

WB analysis using 26939-1-AP

WB analysis using 26939-1-AP

IHC staining of human ovary tumor using 26939-1-AP

IHC staining of human ovary tumor using 26939-1-AP

FC experiment of SH-SY5Y using 26939-1-AP

IF Staining of MCF-7 using 26939-1-AP

IF Staining of NIH/3T3 using 26939-1-AP

WB analysis of HeLa using 26939-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.

Transgelin Promotes Glioblastoma Stem Cell Hypoxic Responses and Maintenance Through p53 Acetylation

Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.

Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI

Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.

ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK