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RAD21 Polyclonal antibody

RAD21 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27071-1-AP

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Synonyms

hHR21, HR21, HRAD21, KIAA0078, MCD1, Nuclear matrix protein 1, NXP 1, NXP1, RAD21, RAD21 homolog (S. pombe), SCC1, SCC1 homolog



经过测试的应用

Positive WB detected inA549 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27071-1-AP targets RAD21 in WB, IHC, IF, ELISA applications and shows reactivity with human, canine samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsIHC, WB
Tested Reactivity human, canine
Cited Reactivityhuman
Immunogen RAD21 fusion protein Ag25481 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAD21 homolog (S. pombe)
Synonyms hHR21, HR21, HRAD21, KIAA0078, MCD1, Nuclear matrix protein 1, NXP 1, NXP1, RAD21, RAD21 homolog (S. pombe), SCC1, SCC1 homolog
Calculated Molecular Weight 631 aa, 72 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC050381
Gene Symbol RAD21
Gene ID (NCBI) 5885
RRIDAB_2880742
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60216
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAD21, also named as Nuclear matrix protein 1, is a 631 amino acid protein, which belongs to the rad21 family. The calculated molecular weight of RAD21 is 72 kDa, but the phosphorylated RAD21 is about 120 kDa. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells

实验方案

Product Specific Protocols
WB protocol for RAD21 antibody 27071-1-APDownload protocol
IHC protocol for RAD21 antibody 27071-1-APDownload protocol
IF protocol for RAD21 antibody 27071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Lett

IQGAP3 interacts with Rad17 to recruit the Mre11-Rad50-Nbs1 complex and contributes to radioresistance in lung cancer.

Authors - Yulan Zeng
humanIHC,WB

Front Oncol

RAD21 Confers Poor Prognosis and Affects Ovarian Cancer Sensitivity to Poly(ADP-Ribose)Polymerase Inhibitors Through DNA Damage Repair.

Authors - Rui Gou
  • KD Validated
humanWB

Cell Death Dis

EGFR-ERK induced activation of GRHL1 promotes cell cycle progression by up-regulating cell cycle related genes in lung cancer.

Authors - Yiming He