Prohibitin Monoclonal antibody
Prohibitin Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, pig, rat and More (1)
Applications
IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
4D3G5
验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue, HeLa cells, human placenta tissue, pig heart tissue, rat heart tissue |
| Positive IHC detected in | human liver tissue, human kidney tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
60092-1-Ig targets Prohibitin in WB, IHC, IF, ELISA applications and shows reactivity with human, pig, rat samples.
| Tested Applications | IHC, WB, ELISA |
| Cited Applications | IF, WB |
| Tested Reactivity | human, pig, rat |
| Cited Reactivity | human, mouse |
| Immunogen | Prohibitin fusion protein Ag1240 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | prohibitin |
| Synonyms | Cell and organelle markers, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, PHB, PHB1, prohibitin |
| Calculated Molecular Weight | 30 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC013401 |
| Gene Symbol | PHB |
| Gene ID (NCBI) | 5245 |
| RRID | AB_2252403 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P35232 |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Prohibitin, also known as PHB, a member of the Band-7 family of proteins, is widely distributed in bacteria, plants, yeast, protozoa and mammals and is important in cell proliferation, differentiation and apoptosis (PMID: 20840588 ). This protein localizes to the inner membrane of mitochondria, where it acts as a chaperone protein, and is also found in the nucleus, where it negatively regulates transcription (PMID: 18558096). Recent study confirmed that the expression and distribution of PHB, which is a nuclear matrix protein, affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis (PMID: 24402549).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Prohibitin antibody 60092-1-Ig | Download protocol |
| IHC protocol for Prohibitin antibody 60092-1-Ig | Download protocol |
| IF protocol for Prohibitin antibody 60092-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Chron Obstruct Pulmon Dis Prohibitin Protects Pulmonary Microvascular Endothelial Cells Against Cigarette Smoke Extract-Induced Cell Apoptosis and Inflammation. | ||
Oncotarget Prohibitin, relocated to the front ends, can control the migration directionality of colorectal cancer cells. |
