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P53 Monoclonal antibody

P53 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse and More (5)

Applications

IF/ICC, IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Publications

182

CloneNo.

6C4B6

Cat No : 60283-2-Ig

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Synonyms

Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

验证数据展示

WB analysis of A431 using 60283-2-Ig
A431 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 60283-2-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
WB analysis of Neuro-2a using 60283-2-Ig
Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 60283-2-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of HSC-T6 using 60283-2-Ig
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of ROS1728 using 60283-2-Ig
ROS1728 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 60283-2-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 60283-2-Ig
mouse colon tissue were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of rat thymus using 60283-2-Ig
rat thymus tissue were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IF Staining of A431 using 60283-2-Ig
Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (60283-2-Ig, Clone: 6C4B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Cat No. 60283-2-Ig

Size

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经过测试的应用

Positive WB detected in	Neuro-2a cells, A431 cells, HSC-T6 cells, ROS1728 cells, A375 cells, COLO 320 cells, mouse colon tissue, rat thymus tissue, HaCaT cells, HT-29 cells, HEK-293 cells, Sp2/0
Positive IHC detected in	human colon cancer tissue, human lung cancer tissue, human breast cancer tissue, human stomach cancer tissue, human tonsillitis tissue, human gliomas tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	A431 cells

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:20000
Immunohistochemistry (IHC)	IHC : 1:1600-1:6400
Immunofluorescence (IF)	IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 5 publications below
WB	See 162 publications below
IHC	See 20 publications below
IF	See 20 publications below
IP	See 7 publications below
CoIP	See 3 publications below

产品信息

60283-2-Ig targets P53 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications	IF/ICC, IHC, WB, ELISA
Cited Applications	WB, IP, IF, IHC, CoIP
Tested Reactivity	Human, rat, mouse
Cited Reactivity	human, rat, rabbit, zebrafish, pig, canine
Immunogen	P53 fusion protein Ag0698 种属同源性预测
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Full Name	tumor protein p53
Synonyms	Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53
Calculated Molecular Weight	44 kDa
Observed Molecular Weight	53 kDa
GenBank Accession Number	BC003596
Gene Symbol	TP53
Gene ID (NCBI)	7157
RRID	AB_2881401
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P04637
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

1. What is p53?

P53 is a tumor suppressor gene that plays a role in maintaining genomic stability and controlling apoptosis. During the cell cycle, it can arrest cells at the G1/S checkpoint and activate DNA repair mechanisms. It is the most mutated gene in cancer. In unstressed cells, p53 usually exists at low levels in an inactive form, being bound to Mdm2.

2. FAQs and p53

a. I fail to detect p53 by western blotting

Basal levels of wild-type p53 in untreated cells can be low. Try to load more cell lysate and use a positive control - a lysate of cells treated with DNA-damaging agents should increase p53 levels.

b. I fail to detect p53 in some cell lines by western blotting

Various p53 mutations are present in cancer cell types. If mutations cause truncations/deletions some monoclonal antibodies may no longer recognize mutated p53. You have more chances of detecting various p53 mutants with our polyclonal antibody.

c. I can detect more than one band ~50 kDa size / different cell lines give bands at slightly different size

p53 is a subject of post-translational modifications (http://p53.free.fr/p53_info/p53_modifications.html) and more than one isoform may be expressed (http://p53.free.fr/p53_info/p53_isoforms.html). Also, it is possible that your cell line of interest expresses one allele with mutated p53 with altered molecular weight.

实验方案

Product Specific Protocols
WB protocol for P53 antibody 60283-2-Ig	Download protocol
IHC protocol for P53 antibody 60283-2-Ig	Download protocol
IF protocol for P53 antibody 60283-2-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Bioact Mater Chemo-immunotherapy by dual-enzyme responsive peptide self-assembling abolish melanoma Authors - Yuhan Wang View Article
mouse	WB	Nat Commun Circular RNA encoded MET variant promotes glioblastoma tumorigenesis Authors - Jian Zhong View Article
mouse	WB	Redox Biol Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice Authors - Boying Zhao View Article
mouse	IHC	Aging (Albany NY) Whole-transcriptome sequencing analysis reveal mechanisms of Yiqi Huoxue Yangyin (YHY) decoction in ameliorating D-gal-induced cardiac aging Authors - Xue Wang View Article
rat	WB,IF	Redox Biol SSBP1 drives high fructose-induced glomerular podocyte ferroptosis via activating DNA-PK/p53 pathway. Authors - Wen-Yuan Wu View Article
pig	WB	J Cachexia Sarcopenia Muscle MyD88 deficiency ameliorates weight loss caused by intestinal oxidative injury in an autophagy-dependent mechanism. Authors - Ming Qi View Article

P53 Monoclonal antibody

P53 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

验证数据展示

WB analysis of A431 using 60283-2-Ig

IHC staining of human colon cancer using 60283-2-Ig

WB analysis of Neuro-2a using 60283-2-Ig

WB analysis using 60283-2-Ig

WB analysis of HSC-T6 using 60283-2-Ig

WB analysis of ROS1728 using 60283-2-Ig

WB analysis using 60283-2-Ig

WB analysis of mouse colon using 60283-2-Ig

WB analysis of rat thymus using 60283-2-Ig

IF Staining of A431 using 60283-2-Ig

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Chemo-immunotherapy by dual-enzyme responsive peptide self-assembling abolish melanoma

Circular RNA encoded MET variant promotes glioblastoma tumorigenesis

Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice

Whole-transcriptome sequencing analysis reveal mechanisms of Yiqi Huoxue Yangyin (YHY) decoction in ameliorating D-gal-induced cardiac aging

SSBP1 drives high fructose-induced glomerular podocyte ferroptosis via activating DNA-PK/p53 pathway.

MyD88 deficiency ameliorates weight loss caused by intestinal oxidative injury in an autophagy-dependent mechanism.