Various lysates were subjected to SDS PAGE followed by western blot with 10306-1-AP (B23/NPM1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10306-1-AP (B23/NPM1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10306-1-AP (B23/NPM1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
WB result of anti-NPM1 (10306-1-AP) in different cell lysates.
IP Result of anti-B23 (IP:10306-1-AP, 4ug; Detection:10306-1-AP 1:2000) with Jurkat cells lysate 3200ug.
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using B23/NPM1 antibody (10306-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10306-1-AP (B23 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).