Various lysates were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

WB result of EGFR antibody (66455-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EGFR transfected HepG2 cells.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human EGFR (66455-1-Ig, Clone:2A2H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

A431 cells were subjected to SDS PAGE followed by western blot with 51071-2-AP (EGFR (C-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IP result of anti-EGFR (C-terminal)(IP:51071-2-AP, 4ug; Detection:51071-2-AP 1:30000) with A431 cells lysate 1200 ug.

Immunofluorescent analysis of (4% PFA) fixed A431 cells using EGFR (C-terminal) antibody (51071-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Standard curve of Human EGFR ELISA Kit KE00294.

Standard curve of Human EGFR ELISA Kit KE00294.

1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human EGFR (CL488-66455, Clone:2A2H10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 EGFR antibody (CL488-30139) at dilution of 1:200.

1x10^6 A431 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 EGFR Polyclonal Antibody (CL488-30139)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were not fixed.

1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human EGFR (CL594-66455, Clone:2A2H10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

WB result of EGFR antibody (66455-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EGFR transfected HepG2 cells. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human EGFR (66455-1-Ig, Clone:2A2H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.