Various lysates were subjected to SDS PAGE followed by western blot with 15967-1-AP (ERp57/ERp60 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

IP result of anti-ERp57/ERp60 (IP:15967-1-AP, 4ug; Detection:15967-1-AP 1:2000) with mouse liver tissue lysate 4000ug.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ERp57/ERp60 antibody (15967-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15967-1-AP (ERp57/ERp60 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15967-1-AP (ERp57/ERp60 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 HepG2 cells were stained with 0.2ug ERp57/ERp60 antibody (15967-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human ERp57/ERp60 (CL647-15967) (red), or 0.2 ug isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).