Various lysates were subjected to SDS PAGE followed by western blot with 66365-1-Ig (ERp72 antibody) at dilution of 1:4900 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66365-1-Ig (ERp72 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 66365-1-Ig (ERp72 antibody) at dilution of 1:4900 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66365-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66365-1-Ig (ERp72 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66365-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 14712-1-AP (ERp72 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14712-1-AP (ERp72 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14712-1-AP (ERp72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using ERp72 antibody (14712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human ERp72 (14712-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

IP result of anti-ERp72 (IP:14712-1-AP, 4ug; Detection:14712-1-AP 1:500) with HeLa cells lysate 2800ug.

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66365 (ERp72 antibody) at dilution of 1:100.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 ERp72 antibody (CL594-66365, Clone: 1D5F3 ) at dilution of 1:200.

Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using CoraLite® Plus 488 ERp72 antibody (CL488-14712) at dilution of 1:200.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using KHC0584 (PDIA4/ERP72 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using KHC0584 (PDIA4/ERP72 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using KHC0584 (PDIA4/ERP72 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using KHC0584 (PDIA4/ERP72 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using KHC0584 (PDIA4/ERP72 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using KHC0584 (PDIA4/ERP72 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using KHC0584 (PDIA4/ERP72 IHC Kit).

ERp72 fusion protein Ag6431 SDS-PAGE