Purity of recombinant human Noggin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human Noggin (HZ-1118-GMP) inhibits dose-dependent induction of alkaline phosphatase production by BMP-4 in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human Noggin and 40 ng/mL of BMP-4 (HZ-1045) for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 1.5-15 ng/mL.

Purity of recombinant human Noggin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human Noggin (HZ-1118-GMP) inhibits dose-dependent induction of alkaline phosphatase production by BMP-4 in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human Noggin and 40 ng/mL of BMP-4 (HZ-1045) for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 3-15 ng/mL.