Recombinant human G-CSF (HZ-1207) stimulates dose-dependent proliferation of the M-NFS-60 Mouse Myeloid Leukemia indicator cells line. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. M-NFS-60 cells were treated with increasing concentrations of recombinant human G-CSF for 72 hours. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 value range is 0.009-0.05 ng/mL.