Purity of recombinant human IL-12 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
Recombinant human IL-12 (HZ-1256) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 1-5 ng/mL.
Proteintech IL-12 (HZ-1256) demonstrates equivalent EC50 and alkaline phosphatase induction to leading competitors. Recombinant human IL-12 stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 1-5 ng/mL.