Purity of recombinant human PTN was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human PTN (HZ-1278) stimulates dose-dependent proliferation of the THP-1 monocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. THP-1 cells were treated with increasing concentrations of recombinant PTN for 120 hours. The EC50 range is 100-500 ng/mL​.
 

Purity of recombinant human PTN was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP-grade recombinant human PTN (HZ-1278) stimulates dose-dependent proliferation of the THP-1 monocyte cell line. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. THP-1 cells were treated with increasing concentrations of recombinant PTN for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 120-650 ng/mL​.