Various lysates were subjected to SDS PAGE followed by western blot with 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66220-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66220-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66220-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PD-1/CD279 mouse mAb (66220-1-Ig) at dilution of 1:50 and CD20 rabbit pAb (24828-1-AP) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66220-1-Ig, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) for 24828-1-AP. This data was developed using the same antibody clone with 66220-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PD-1/CD279 antibody (66220-1-Ig, Clone: 4H4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66220-1-PBS in a different storage buffer formulation.
1X10^6 unstimulated (dashed line) or PMA and ionomycin treated (red) MOLT-4 cells were surface stained with 0.2 ug Anti-Human PD-1/CD279 (66220-1-Ig, Clone: 4H4D1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or 0.2 ug isotype control antibody (blue, solid line). Cells were not fixed. This data was developed using the same antibody clone with 66220-1-PBS in a different storage buffer formulation.